Glutamate Receptor 1 抗体 (Extracellular)
Quick Overview for Glutamate Receptor 1 抗体 (Extracellular) (ABIN7043224)
抗原
See all Glutamate Receptor 1 (GLUR1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 271-285, Extracellular
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原理
- A Guinea Pig Polyclonal Antibody to AMPA-Selective Glutamate Receptor 1 (GluR1)
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human,pig,dog - 13,14 amino acid residues identical
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产品特性
- Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody is directed against an extracellular epitope the rat ionotropic glutamate receptor 1. Guinea pig Anti-GluR1 (GluA1) (extracellular) Antibody raised in guinea pig can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize GluR1 from human, mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-GluR1 (GluA1) (extracellular) Antibody (ABIN7043226, ABIN7044328 and ABIN7044329) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: RTSDSRDHTRVDWKR(C), corresponding to amino acid residues 271-285 of rat GluR1
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亚型
- IgG
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anti-Glutamate Receptor 1 (GLUR1) (AA 19-300) antibody
GLUR1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor 1 (GLUR1) (pSer845) antibodyGLUR1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor 1 (GLUR1) (AA 19-360) antibodyGLUR1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor 1 (GLUR1) antibodyMicroarray verified GLUR1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB177574 unconjugated
anti-Glutamate Receptor 1 (GLUR1) (C-Term) antibodyGLUR1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor 1 (GLUR1) (pSer831) antibodyGLUR1 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Receptor 1 (GLUR1) (AA 1-389) antibodyGLUR1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S355-1 unconjugated
anti-Glutamate Receptor 1 (GLUR1) (AA 1-389) antibody (HRP)GLUR1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S355-1 HRP
anti-Glutamate Receptor 1 (GLUR1) (AA 1-389) antibody (FITC)GLUR1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S355-1 FITC
anti-Glutamate Receptor 1 (GLUR1) (AA 1-389) antibody (Biotin)GLUR1 适用: 大鼠 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal S355-1 Biotin
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235619)
Blocking Peptide: (ABIN7235619)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Glutamate Receptor 1 (GLUR1)
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别名
- GRIA1
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背景
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AMPA Receptor 1, Glutamate receptor 1, Ionotropic glutamate receptor 1, AMPA-selective glutamate receptor 1, GRIA1, GluR-A, GluR-K1,AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of an arginine (R) at a critical site in the pore loop instead of a glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.
Alternative names: GluR1 (GluA1), AMPA Receptor 1, Glutamate receptor 1, Ionotropic glutamate receptor 1, AMPA-selective glutamate receptor 1, GRIA1, GluR-A, GluR-K1 -
基因ID
- 50592
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NCBI登录号
- NM_000827
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UniProt
- P19490
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途径
- PI3K-Akt Signaling
抗原
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