GJC1 抗体 (AA 153-166)
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- 抗原 See all GJC1 抗体
- GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))
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抗原表位
- AA 153-166
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GJC1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to Connexin-45
- 特异性
- Intracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Mouse,human - identical
- 产品特性
- Anti-Connexin-45 (GJC1) Antibody is directed against an epitope of rat Connexin-45. Anti-Connexin-45 (GJC1) Antibody (ABIN7043207, ABIN7044085 and ABIN7044086)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx45 from human, rat and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KEQSQPKPKHDGRR, corresponding to amino acid residues 153-166 of rat Connexin-45
- 亚型
- IgG
- Top Product
- Discover our top product GJC1 Primary Antibody
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IP
Negative Control: BLP-CC207
Blocking Peptide: BLP-CC207
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- GJC1 (Gap Junction Protein, gamma 1, 45kDa (GJC1))
- 别名
- GJC1 (GJC1 产品)
- 背景
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CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7,Gap junctions are plasma membrane domains of intercellular channels that allow direct connection between the cytoplasm of adjacent cells. They allow exchange of low molecular weight metabolites, ions and small hydrophilic molecules. These channels are formed by head-to-head docking of hexameric hemichannels called connexons, whose subunit proteins are encoded by the connexin (Cx) gene family1-3.The connexin subunit is formed of four-transmembrane spanning domains, harboring two extracellular loops, one cytoplasmic loop and cytoplasmic N- and C-terminal regions. To date, the family of connexin comprises 21 genes in the human genome4.Connexin 45 (Cx45), encoded by the GJC1 gene, is expressed in cardiomyocytes and in developing vasculature in both endothelial and smooth muscle cells4,5.In the heart, Cx45 along with Cx40 and Cx43, play a crucial role in normal electrical conduction and synchronous heart muscle contraction and important for the normal embryonic development of the heart6. Indeed Cx45 forms low conductance and voltage-sensitive gap junctions between the ventricular cardiac myocytes and the core conduction system2. Studies have showed that cardiac connexin Cx45 is upregulated in human failing hearts and has an important role in the regulation of endothelial-induced mural cell differentiation4,5.
Alternative names: Connexin-45 (GJC1), CX45, Gap junction gamma1 protein, Gap junction alpha7 protein, GJA7 - 基因ID
- 266706
- NCBI登录号
- NM_005497
- UniProt
- A4GG66
- 途径
- Cell-Cell Junction Organization
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