GABARAP 抗体 (Intracellular)
Quick Overview for GABARAP 抗体 (Intracellular) (ABIN7043179)
抗原
See all GABARAP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-15, Intracellular
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原理
- A Rabbit Polyclonal Antibody to GABA Receptor Associated Protein
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特异性
- Intracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize:GABA receptor associated protein-like 1 (11/14 amino acid residues identical), (Accession Q0VGK0).GABA receptor associated protein-like 3 (11/14 amino acid residues identical), (Accession Q9BY60).May recognize GABA receptor associated protein-like 2 (8/14 amino acid residues identical), (Accession P60522).
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预测反应
- Mouse,human - identical
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产品特性
- Anti-GABARAP Antibody (ABIN7043179, ABIN7044532 and ABIN7044533) is a highly specific antibody directed against an extracellular epitope of the rat GABA receptor associated protein. The antibody can be used in western blot analysis. It has been designed to recognize GABARAP from human, mouse and rat samples. Due to high homology with GABA receptor associated protein-like proteins the antibody will recognize GABA receptor associated protein 1 and 3 and may recognize GABA receptor associated protein 2.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KFVYKEEHPFEKRR, corresponding to amino acid residues 2-15 of rat GABARAP
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亚型
- IgG
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anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-100) antibody
GABARAP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-30) antibodyGABARAP 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11846 unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-30) antibodyGABARAP 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-31), (N-Term) antibodyGABARAP 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-117) antibodyGABARAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibodyGABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (HRP)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (FITC)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (Biotin)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (PE)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal PE
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000
Application Dilutions Western blot wb: 1:800
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说明
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Negative Control: (ABIN7235469)
Blocking Peptide: (ABIN7235469)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))
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别名
- GABARAP
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背景
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GABA receptor associated protein, GABARAPL,GABA(A) receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABA associated protein (GABARAP) family interacts specifically with the γ1, γ2S and γ2L subunits of the GABA(A) receptor. GABARAP is expressed in a variety of tissues including heart, brain, placenta, liver and others. The genetic sequence encoding GABARAP is similar to microtubule-associated proteins 1A and 1B1.GABARAP has an asymmetric structure with a basic N-terminal region and an acidic C terminus. GABARAP has a compact fold consisting of four or five-stranded β-sheets with two α-helices on each side. The outer strands of the β-sheets are aligned anti-parallel to the inner strands and helices α3 and α4 are located on one side of the sheet. The presence of α1 and α2 is a unique feature of GABARAP and forms a hydrophilic patch suitable for protein-protein interface. GABARAP exists in a monomeric conformation at low protein concentration and a head-to-tail oligomeric conformation in high salt conditions.GABARAP is involved in intracellular trafficking and is an integral part of the intra-Golgi transport machinery. Thus it is detected in cellular compartments such as the Golgi apparatus and endoplasmic reticulum. GABARAP affects GABA(A) receptors' cell surface expression by increasing their intracellular trafficking. Disruption of cellular microtubules prevents the clustering of both GABARAP and GABA(A) receptors. GABARAP also interacts with other receptors such as the glycine receptor at the postsynaptic site2.
Alternative names: GABARAP, GABA receptor associated protein, GABARAPL -
基因ID
- 58974
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NCBI登录号
- NM_007278
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UniProt
- P60517
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途径
- Autophagy
抗原
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