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GPR84 抗体 (Extracellular)

Name: GPR84 抗体 (Extracellular)
SKU: ABIN7043175
Availability: OutOfStock

GPR84 适用: 人 WB, IHC, IF, LCI, IF (cc) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human U-87 MG glioblastoma (lanes 3 and 6) cell line lysates: - 1-3. (GPR84 抗体 (Extracellular))

Expression of GPR84 in mouse fornix in a kainic acid-induced model of temporal lobe epilepsy - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444), (1:200), followed by goat anti-rabbit-AlexaFluor-488. (GPR84 抗体 (Extracellular))

IHC

Cell surface detection of GPR84 in live intact mouse J774 macrophage cells: (black line) Cells. (GPR84 抗体 (Extracellular))

FACS

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6): - 1-3. (GPR84 抗体 (Extracellular))

4 images

产品编号 ABIN7043175

发货至: 中国

Datasheet as PDF

抗原 See all GPR84 抗体

GPR84 (G Protein-Coupled Receptor 84 (GPR84))
抗原表位
16

7

5

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 166-180, Extracellular
适用
31

22

4

3

3

1

1

1

1

1

1
人
宿主
45

3
兔
Compatible Secondaries
克隆类型
46

2
多克隆
标记
19

5

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GPR84 antibody is un-conjugated
应用范围
35

22

20

6

5

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

原理

A Rabbit Polyclonal Antibody to GPR84

特异性

2nd extracellular loop

交叉反应

人, 小鼠, 大鼠

预测反应

Human - 14,15 amino acid residues identical, mouse,rat - 13

产品特性

Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

亚型

IgG

Top Product

Discover our top product GPR84 Primary Antibody
anti-G Protein-Coupled Receptor 84 (GPR84) (AA 21-120) antibody
GPR84 适用: 人, 小鼠, 兔 WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 适用: 人, 马, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 适用: 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) antibody
GPR84 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200

说明

Cited Application: IP
Negative Control: BLP-GR052
Blocking Peptide: BLP-GR052

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

别名

GPR84 (GPR84 产品)

背景

G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33,GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Alternative names: GPR84, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

基因ID

53831

NCBI登录号

NM_020370

UniProt

Q9NQS5