Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) 抗体
Quick Overview for Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) 抗体 (ABIN7043165)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 86-109, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR56
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 （详细）
- This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.
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预测反应
- 14 amino acid residues identical,Mouse - 13, human - 11
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产品特性
- Anti-GPR56 (extracellular) Antibody (ABIN7043165, ABIN7044433 and ABIN7044434) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56
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亚型
- IgG
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anti-Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 26-216) antibody
ADGRG1 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (Internal Region) antibodyADGRG1 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) antibody (FITC)ADGRG1 适用: 大鼠 FACS 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235431)
Blocking Peptide: (ABIN7235431)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Adhesion G Protein-Coupled Receptor G1 (ADGRG1)
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别名
- ADGRG1
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背景
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G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1,GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present1-3.Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo1-3.Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain2,3.
Alternative names: GPR56, G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1 -
基因ID
- 260326
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NCBI登录号
- NM_001145770
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UniProt
- Q8K3V3
抗原
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