GPR4 抗体 (Extracellular)
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- 抗原 See all GPR4 抗体
- GPR4 (G Protein-Coupled Receptor 4 (GPR4))
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抗原表位
- AA 162-177, Extracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPR4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))
- 原理
- A Rabbit Polyclonal Antibody to GPR4
- 特异性
- 2nd extracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat,mouse,human - 15,16 amino acid residues identical
- 产品特性
- Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GPR4 from mouse, rat, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4
- 亚型
- IgG
- Top Product
- Discover our top product GPR4 Primary Antibody
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anti-G Protein-Coupled Receptor 4 (GPR4) (C-Term) antibody
GPR4 适用: 人, 犬, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibodyGPR4 适用: 人, 大鼠, 小鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 285-362) antibodyGPR4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Cytoplasmic Domain) antibodyGPR4 适用: 人, 犬, Pig, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Extracellular Domain) antibodyGPR4 适用: 人, 大鼠, 小鼠, 犬, Pig, Cow, 猴, Bat, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (Internal Region) antibodyGPR4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 201-250) antibodyGPR4 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 4 (GPR4) (AA 196-224) antibodyGPR4 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB43856 unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: (ABIN7235415)
Blocking Peptide: (ABIN7235415)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- GPR4 (G Protein-Coupled Receptor 4 (GPR4))
- 别名
- GPR4 (GPR4 产品)
- 背景
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Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.
Alternative names: GPR4, Acid-sensing G-protein coupled receptor 4, GPR19 - 基因ID
- 308408
- NCBI登录号
- NM_005282
- UniProt
- Q4KLH9
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