GPR35 抗体 (Extracellular)
Quick Overview for GPR35 抗体 (Extracellular) (ABIN7043156)
抗原
See all GPR35 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 156-170, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR35 (extracellular)
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特异性
- Extracellular, 2nd loop.
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Not recommended for human samples.
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预测反应
- Mouse - 14 out of 15 amino acid residues identical, rat - 12 out of 15 amino acid residues identical Not recommended for human samples
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产品特性
- Anti-GPR35 (extracellular) Antibody (ABIN7043156, ABIN7044441 and ABIN7044442) is a highly specific antibody directed against an extracellular epitope of the mouse GPR35. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR35 from mouse and rat samples. The antibody will not recognize the receptor from human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EGGF(S)FSSQTRRNFS, corresponding to amino acid residues 156 - 170 of mouse GPR35
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亚型
- IgG
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anti-G Protein-Coupled Receptor 35 (GPR35) (Internal Region) antibody
GPR35 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (Extracellular Domain) antibodyGPR35 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (AA 240-309) antibodyGPR35 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (Internal Region) antibodyGPR35 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (AA 275-325) antibodyGPR35 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (AA 51-100) antibodyGPR35 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 35 (GPR35) (AA 156-170), (Extracellular) antibody (FITC)GPR35 适用: 小鼠 FACS, LCI 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235400)
Blocking Peptide: (ABIN7235400)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR35 (G Protein-Coupled Receptor 35 (GPR35))
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别名
- GPR35
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背景
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G-Protein Coupled Receptor 35, Kynurenic Acid Receptor,G- protein coupled receptor 35 (GPR35) is an "orphan" GPCR, whose native ligand is still unknown. It was shown to be involved in the modulation of synaptic transmission, nociception, neuropathic and inflammatory pain, and in various diseases including ulcerative colitis and primary sclerosing cholangitis1 and hence there is a great therapeutic potential in studying its activity.In vitro, GPR35 is stimulated by high concentrations of a number of small molecules, including kynurenic acid, 2-oleoyllysophosphatidic acid, DHICA, reverse T3, cGMP and it has a wide range of synthetic agonists and antagonists that either activate or inactivate it, respectively (Summarized in2). GPR35 is composed of seven trans-membrane domains, and it was shown to share structural homology with various receptors for chemokines, chemotactic cytokines that direct the traffic of leukocytes and other cells in the body. It was shown to be activated by chemokine CXCL17, and hence the role of a chemokine receptor was also assigned to it3.GPR35 expression has been identified within discrete regions of the central nervous system (CNS) and peripheral nervous system, and lower levels in heart, lung and skeletal muscle, and also in the lower gut and colon and by various white cell groups, including numerous dendritic cell and monocyte populations4.
Alternative names: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor -
基因ID
- 64095
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NCBI登录号
- NM_001195382
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UniProt
- Q9ES90
抗原
-
