GPR34 抗体 (Extracellular)
Quick Overview for GPR34 抗体 (Extracellular) (ABIN7043153)
抗原
See all GPR34 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 197-209, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR34
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特异性
- 2nd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Human,rat - 12,13 amino acid residues identical
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产品特性
- Anti-GPR34 (extracellular) Antibody (ABIN7043153, ABIN7044449 and ABIN7044450) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34
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亚型
- IgG
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anti-G Protein-Coupled Receptor 34 (GPR34) (AA 1-61) antibody
GPR34 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) antibodyGPR34 适用: 人, 马, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (AA 1-381) antibodyGPR34 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (C-Term) antibodyGPR34 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (AA 232-261) antibodyGPR34 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32575 unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (Internal Region) antibodyGPR34 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (AA 181-230) antibodyGPR34 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (Center) antibodyGPR34 适用: 人, 大鼠, 猴, Pig WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (AA 181-230) antibodyGPR34 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 34 (GPR34) (AA 197-209), (Extracellular) antibody (FITC)GPR34 适用: 小鼠 FACS 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235396)
Blocking Peptide: (ABIN7235396)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR34 (G Protein-Coupled Receptor 34 (GPR34))
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别名
- GPR34
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背景
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G-protein coupled receptor 34, LYPSR1,The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system-derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies shows that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages, GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients, GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.
Alternative names: G-protein coupled receptor 34 -
基因ID
- 23890
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NCBI登录号
- NM_005300
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UniProt
- Q9R1K6
抗原
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