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GPR34 抗体

GPR34 适用: 人, 马, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1048802
发货至: 中国
  • 抗原 See all GPR34 抗体
    GPR34 (G Protein-Coupled Receptor 34 (GPR34))
    适用
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    人, 马, Cow
    宿主
    • 54
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    克隆类型
    • 56
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    多克隆
    标记
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    This GPR34 antibody is un-conjugated
    应用范围
    • 28
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    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    预测反应
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Bovine, Horse (100%) Marmoset, Mouse, Rat, Elephant, Guinea pig (94%) Panda (88%) Bat, Opossum (82%).
    纯化方法
    Immunoaffinity purified
    Top Product
    Discover our top product GPR34 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (2.5 - 5 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    GPR34 (G Protein-Coupled Receptor 34 (GPR34))
    别名
    GPR34 (GPR34 产品)
    别名
    GPR34 antibody, fl10e05 antibody, wu:fl10e05 antibody, zgc:110315 antibody, G protein-coupled receptor 34 antibody, G protein-coupled receptor 34a antibody, G protein-coupled receptor 34b antibody, GPR34 antibody, Gpr34 antibody, gpr34a antibody, gpr34b antibody
    背景
    Name/Gene ID: GPR34
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: GPR34, G protein-coupled receptor 34
    基因ID
    2857
    UniProt
    Q9UPC5
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