Mesothelin 抗体 (Extracellular)
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- 抗原 See all Mesothelin (MSLN) 抗体
- Mesothelin (MSLN)
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抗原表位
- AA 294-307, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Mesothelin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))
- 原理
- A Rabbit Polyclonal Antibody to GPER1/GPR30
- 特异性
- 3rd extracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat - identical, human - 13,14 amino acid residues identical
- 产品特性
- Anti-GPER1/GPR30 (extracellular) Antibody (ABIN7043145, ABIN7044265 and ABIN7044266)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPER1 from rat, mouse, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CKQSFRHAYPLTGH, corresponding to amino acid residues 294 - 307 of mouse GPER1
- 亚型
- IgG
- Top Product
- Discover our top product MSLN Primary Antibody
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anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal MSLN-2131 unconjugated
anti-Mesothelin (MSLN) (AA 101-200) antibodyMSLN 适用: 人 ELISA, WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 273-407) antibodyMSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人 ELISA, WB, FACS 宿主: 小鼠 Monoclonal 5C11A4 unconjugated
anti-Mesothelin (MSLN) (AA 37-598) antibodyMSLN 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 296-580) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM71 unconjugated Recombinant Antibody
anti-Mesothelin (MSLN) (AA 296-606) antibodyMSLN 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 7E6A6 unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4H12F8 unconjugated
anti-Mesothelin (MSLN) (AA 1-62) antibodyMSLN 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) antibodyMSLN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IFC
Negative Control: BLP-ER049
Blocking Peptide: BLP-ER049
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- Mesothelin (MSLN)
- 别名
- GPER1 (MSLN 产品)
- 背景
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G protein-coupled estrogen receptor 1, G protein-coupled receptor 30, Membrane estrogen receptor, Chemoattractant receptor-like 2, GPER,GPER1/GPR30 is an estrogen receptor belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members, GPER1 has seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal domain. It is widely expressed in the central nervous system (CNS) and it binds 17β-estradiol (17β-E) with high affinity1.Estrogens have long been known as important regulators of the female reproductive functions, however, is now commonly accepted that estrogens and androgens have important functions in both female and male physiology and pathology.As opposed to the epigenetic regulatory role in estrogen signaling carried out by ER-α and ER-β estrogen receptors, signaling from GPER1 is responsible for the rapid non-genomic actions of estrogens that activate various protein-kinase cascades1. GPER1 signaling improves spatial memory, possibly via release of neurotransmitters. In addition, GPER1 activation contributes to behaviors that denote anxiety, and to social behaviors such as social memory and lordosis behavior in mice. In the male hippocampus, GPER1 activation leads to phosphorylation of the classical estrogen receptor ERα, suggesting that crosstalk with ERα is important in the manifestation of these behaviors, many of which are absent in ERα-null mice2. GPER1 activation also has several beneficial effects in the cardiovascular system including vasorelaxation, inhibition of smooth muscle cell proliferation, and protection of the myocardium against ischemia/reperfusion injury, and in the metabolic system including stimulation of insulin release and protection against pancreatic β-cell apoptosis. Thus, GPER1 is emerging as a candidate therapeutic target in both cardiovascular and metabolic diseases3.
Alternative names: G protein-coupled estrogen receptor 1, G protein-coupled receptor 30, Membrane estrogen receptor, Chemoattractant receptor-like 2, GPER - 基因ID
- 76854
- NCBI登录号
- NM_001039966
- UniProt
- Q8BMP4
- 途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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