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Mesothelin 抗体 (Intracellular)

Name: Mesothelin 抗体 (Intracellular)
SKU: ABIN7043144
Availability: OutOfStock

MSLN 适用: 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Expression of GPER in rat dentate gyrus - Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-GPER (GPR30) Antibody (ABIN7043144, ABIN7044267 and ABIN7044268). (Mesothelin 抗体 (Intracellular))

IHC

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat lung (lanes 3 and 6) membranes: - 1-3. (Mesothelin 抗体 (Intracellular))

2 images

产品编号 ABIN7043144

发货至: 中国

Datasheet as PDF

抗原 See all Mesothelin (MSLN) 抗体

Mesothelin (MSLN)
抗原表位
16

6

5

5

5

5

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 329-342, Intracellular
适用
104

20

5

2

2

2

1

1
大鼠
宿主
81

30

4

2

1

1
兔
Compatible Secondaries
克隆类型
77

39

3
多克隆
标记
78

10

8

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Mesothelin antibody is un-conjugated
应用范围
73

62

37

27

13

12

11

5

4

4

4

2

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

原理

A Rabbit Polyclonal Antibody to G-Protein Coupled Estrogen Receptor 1

特异性

Intracellular, C-terminus

交叉反应

小鼠, 大鼠

预测反应

Mouse - 13,14, human - 12,14 amino acid residues identical

产品特性

Anti-GPER (GPR30) Antibody (ABIN7043144, ABIN7044267 and ABIN7044268)) is a highly specific antibody directed against an epitope of rat G-protein coupled estrogen receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPER from rat, mouse and human samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER

亚型

IgG

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应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200

说明

Negative Control: BLP-ER050
Blocking Peptide: BLP-ER050

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

Mesothelin (MSLN)

别名

GPER1 (MSLN 产品)

背景

G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2,The G protein-coupled estrogen receptor (GPER), formerly known as G protein receptor 30 (GPR30), was identified as a novel estrogen receptor that mediates a rapid, non-genomic response to estrogens1.GPER is a G protein-coupled seven-transmembrane receptor. It is believed that the N-terminus is located outside of the cell, and that aspartic acid residues in the terminal region might be modified by glycosylation if GPER is localized in the plasma membrane. It is speculated that the ligand associates with the N-terminal domain to activate the receptor. Trimeric G protein is presumed to bind to the 3rd loop of the intracellular domain based on the molecular structure. A PDZ domain appears to be in the C-terminal region of GPER, but its physiological role is unknown2.GPER is widely distributed in the human body including: brain, liver, heart, lung, pancreas, placenta, blood vessels, bone, lymphoid tissue, as well as endometrial, ovarian and breast cancer. The receptor has been localized both in the plasma membrane and in the endoplasmic reticulum3.It was found that GPER is rapidly induced in the cardiomyocyte ischemia model, and that its induction is associated with apoptotic cell death during ischemia and reperfusion4. In addition, the GPER mediates estrogenic signaling in breast cancer cells and cancer-associated fibroblasts (CAF) that contribute to cancer progression5.

Alternative names: GPER (GPR30), G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2

基因ID

171104

NCBI登录号

NM_001039966

UniProt

O08878

途径

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process