FFAR2 抗体 (Extracellular)
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Quick Overview for FFAR2 抗体 (Extracellular) (ABIN7043139)
抗原
See all FFAR2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 153-167, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)
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特异性
- 2nd extracellular loop
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交叉反应 （详细）
- Not recommended for mouse or rat samples.
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预测反应
- Human only
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产品特性
- Anti-Human GPR43/FFAR2 (extracellular) Antibody (ABIN7043139, ABIN7044283 and ABIN7044284) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2
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亚型
- IgG
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anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 41-140) antibody
FFAR2 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 301-314), (Intracellular) antibodyFFAR2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 277-330) antibodyFFAR2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibodyFFAR2 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (Extracellular Domain) antibodyFFAR2 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (Cytoplasmic Domain) antibodyFFAR2 适用: 人, 小鼠, 大鼠, 猴, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 286-315) antibodyFFAR2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 231-330) antibodyFFAR2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 271-320) antibodyFFAR2 适用: 人 ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibodyFFAR2 适用: 人 IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
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别名
- FFAR2
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背景
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G-protein coupled receptor 43, Free fatty acid receptor 2,Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.
Alternative names: GPR43/FFAR2, G-protein coupled receptor 43, Free fatty acid receptor 2 -
基因ID
- 2867
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NCBI登录号
- NM_005306
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UniProt
- O15552
抗原
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