FFAR2 抗体 (C-Term, Intracellular)
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Quick Overview for FFAR2 抗体 (C-Term, Intracellular) (ABIN7886328)
抗原
See all FFAR2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 301-314, C-Term, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Will not recognize human samples.
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预测反应
- Mouse -12,14 amino acid residues identical
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产品特性
- Anti-GPR43/FFAR2 Antibody (ABIN7043138, ABIN7044289 and ABIN7044290) is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. The antibody can be used in western blot analysis. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat FFAR2
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亚型
- IgG
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anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 41-140) antibody
FFAR2 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibodyFFAR2 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 277-330) antibodyFFAR2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (Extracellular Domain) antibodyFFAR2 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (Cytoplasmic Domain) antibodyFFAR2 适用: 人, 小鼠, 大鼠, 猴, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 271-320) antibodyFFAR2 适用: 人 ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 231-330) antibodyFFAR2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 286-315) antibodyFFAR2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 286-315), (C-Term) antibodyFFAR2 适用: 人 WB 宿主: 兔 Polyclonal RB35768 unconjugated
anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibodyFFAR2 适用: 人 IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200-1:500
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:300
IP: The optimal concentration should be determined by the user
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说明
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Cited Application: IFC
Negative Control: (ABIN7235333)
Blocking Peptide: (ABIN7235333)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
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别名
- FFAR2
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背景
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Synonyms: G-protein coupled receptor 43, Free fatty acid receptor 2
Description: Fatty acids have long been recognized for the variety of their effects in the body. Yet, until recently, these actions were thought to be mediated exclusively via actions on cellular metabolism. There are currently three known receptors for free fatty acids: FFA1, FFA2, and FFA3.Free fatty acid receptor 2 (FFA2) is a G-protein coupled receptor containing seven hydrophobic regions, consistent with transmembrane (TM) spanning helices. FFA2 contains cysteine residues in the first and second extracellular loops that are likely to contribute to structure via the formation of intramolecular disulfide bonds. FFA2 mRNA is detected in a number of tissues, but the highest expression is found in immune cells such as neutrophils, monocytes, peripheral blood mononuclear cells, B-lymphocytes, and polymorphonuclear cells.Short chain fatty acids (SCFAs) are fatty acids of less than six carbons and were found to be endogenous agonists of FFA2. The most potent agonist of the receptor is acetate (C2) and propionate (C3) being equipotent followed by butyrate (C4) and then valerate (C5) and formate (C1)1.FFA2 is implicated in the pathogenesis of several diseases. Stimulation of FFA2 by acetate allows resolution of a colitis-related inflammatory response. FFA2 knockout mice show exacerbated or unresolved inflammation in cases of acute and chronic colitis, arthritis, and asthma. In contrast, other studies show that knockout mice exhibit an increased mortality compared to control mice, despite reduced immune cell recruitment, decreased colonic inflammation, and attenuated colonic tissue damage2.
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基因ID
- 292794
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NCBI登录号
- NM_005306
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UniProt
- Q76EI6
抗原
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