Ephrin B1 抗体 (Extracellular)
Quick Overview for Ephrin B1 抗体 (Extracellular) (ABIN7043131)
抗原
See all Ephrin B1 (EFNB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 208-221, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Ephrin-B1
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特异性
- Extracellular domain
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交叉反应
- 人, 小鼠
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预测反应
- Mouse - identical, rat - 13,14 amino acid residues identical
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产品特性
- Anti-Ephrin-B1 (extracellular) Antibody is directed against an epitope of human Ephrin-B1. Anti-Ephrin-B1 (extracellular) Antibody (ABIN7043131, ABIN7044261 and ABIN7044262) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the ligand in living cells. It has been designed to recognize Ephrin-B1 from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KHETVNQEEKSGPG, corresponding to amino acid residues 208-221 of human Ephrin-B1
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亚型
- IgG
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anti-Ephrin B1 (EFNB1) (AA 28-237) antibody
EFNB1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (pTyr324), (pTyr329) antibodyEFNB1 适用: 人 WB, ELISA, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (pTyr317) antibodyEFNB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (pTyr330) antibodyEFNB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) antibodyEFNB1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (pTyr317) antibodyEFNB1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (Ser312) antibodyEFNB1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (AA 179-228) antibodyEFNB1 适用: 人, 小鼠, 犬, 猴, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (AA 259-346) antibodyEFNB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ephrin B1 (EFNB1) (AA 88-116) antibodyEFNB1 适用: 人 WB 宿主: 兔 Polyclonal RB42526 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235313)
Blocking Peptide: (ABIN7235313)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Ephrin B1 (EFNB1)
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别名
- EFNB1
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背景
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EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1,The erythropoietin-producing hepatocellular (EPH) receptors represent the largest known family of receptor tyrosine kinases and are activated by interactions with cell-surface ligands, termed EFNs. EPH receptors have been classified into two subfamilies, EPHA and EPHB, according to their preference for either glycosylphosphatidylinositol (GPI)-anchored EFN-A ligands or transmembrane EFN-B ligands. Thus far, 14 vertebrate receptors and eight ligands have been identified1. All the ligands share a conserved core sequence of approximately 125 amino acids, including 4 invariant cysteine residues, probably corresponding to a receptor binding domain. This is followed by a membrane anchorage domain, taking the form of a GPI anchor for five of the ligands (ephrin-A1 to -A5) or a transmembrane domain for three of them (ephrin-B1 to -B3) 2.Ephrin-B1 is signals forward or reverse signaling mechanisms. Ephrin-B1 PDZ-dependent reverse signaling controls axon guidance of the corpus callosum, whereas forward signaling is critical for normal craniofacial development2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they are involved in fundamental developmental processes of the nervous system, including axon guidance, axonfasciculation, neural crest cell migration, acquisition of brain subregional identity,and neuronal cell survival. Ephrins are dramatically expressed in a wide range of regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Overexpression of B-type ephrin in cancer cells is reported to correlate with high invasion and high vascularity of tumors and elevated expression of ephrin-B1 is observed in poorly differentiated invasive tumor cells and other tumors with poor clinical prognosis4.
Alternative names: Ephrin-B1, EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1 -
基因ID
- 1947
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NCBI登录号
- NM_004429
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UniProt
- P98172
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途径
- RTK signaling
抗原
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