EFCAB4B 抗体 (Intracellular)
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Quick Overview for EFCAB4B 抗体 (Intracellular) (ABIN7043114)
抗原
See all EFCAB4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 245-258, Intracellular
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原理
- A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A
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交叉反应
- 人, 大鼠
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预测反应
- Rat - 13,14 amino acid residues identical
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产品特性
- Anti-CRACR2A (EFCAB4B) Antibody is directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (ABIN7043114, ABIN7044115 and ABIN7044116) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A
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亚型
- IgG
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anti-EF-Hand Calcium Binding Domain 4B (EFCAB4B) (AA 1-200) antibody
EFCAB4B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-EF-Hand Calcium Binding Domain 4B (EFCAB4B) (AA 1-395) antibodyEFCAB4B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-EF-Hand Calcium Binding Domain 4B (EFCAB4B) (AA 215-264) antibodyEFCAB4B 适用: 人, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-EF-Hand Calcium Binding Domain 4B (EFCAB4B) (AA 71-120) antibodyEFCAB4B 适用: 人, 马, 大鼠, Cow, 犬, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-EF-Hand Calcium Binding Domain 4B (EFCAB4B) (AA 48-74), (N-Term) antibodyEFCAB4B 适用: 人 WB 宿主: 兔 Polyclonal RB38227 unconjugated
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应用备注
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WB: 1:400
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235259)
Blocking Peptide: (ABIN7235259)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))
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别名
- CRACR2A
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背景
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Synonyms: CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B
Description: Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as "CRACs"1.Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.
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基因ID
- 84766
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NCBI登录号
- NM_032680
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UniProt
- Q9BSW2
抗原
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