CRHR1 抗体 (Extracellular)
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Quick Overview for CRHR1 抗体 (Extracellular) (ABIN7043086)
抗原
See all CRHR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 31-44, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- 14 amino acid residues identical, human - 11,Mouse -12
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产品特性
- Anti-CRF1/CRHR1 (extracellular) Antibody (ABIN7043086, ABIN7044185 and ABIN7044186) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1
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亚型
- IgG
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anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 107-117) antibody
Verified CRHR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 24-121) antibodyCRHR1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 24-120) antibodyCRHR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 250-263) antibodyVerified CRHR1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (N-Term) antibodyCRHR1 适用: 人, 大鼠, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (N-Term) antibodyCRHR1 适用: 人, 小鼠, 大鼠, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (AA 55-150) antibodyCRHR1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) antibodyMicroarray verified CRHR1 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB95796 unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (Extracellular Domain) antibodyCRHR1 适用: 人, 犬, 马, 猴, Cow, 绵羊, Hamster, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin Releasing Hormone Receptor 1 (CRHR1) (N-Term) antibodyCRHR1 适用: 人, 小鼠, 大鼠, 犬, 马, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235173)
Blocking Peptide: (ABIN7235173)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))
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别名
- CRHR1
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背景
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Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1,Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic-pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.
Alternative names: CRF1 (CRHR1), Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1 -
基因ID
- 58959
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NCBI登录号
- NM_001145146
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UniProt
- P35353
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途径
- Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity
抗原
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