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CRHR1 抗体 (AA 107-117)

Cited in 3+ publications. The 山羊 多克隆 anti-CRHR1 antibody (ABIN238557) specifically detects CRHR1 in WB, ELISA 和 IF. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN238557
发货至: 中国
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Quick Overview for CRHR1 抗体 (AA 107-117) (ABIN238557)

抗原

See all CRHR1 抗体
CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

适用

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人, 小鼠, 大鼠

宿主

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山羊

克隆类型

  • 90
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多克隆

标记

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This CRHR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    AA 107-117

    原理

    CRHR1 / CRF-R (aa 107 - 117)

    序列

    NEEKKSKVHY H

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NEEKKSKVHYH, from the internal region of the protein sequence according to NP_004373.2.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in Human Brain (Cerebellum), Colon, Rat Brain and Mouse spinal cord lysates (calculated MW of 47.7 kDa according to NP_004373.2). The observed molecular weight corresponds to earlier findings in literature with di

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Expression of the protein seen in the membranes, vesicles and cytoplasm of MCF7 and Neuro2a cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Xie, Penzes, Srivastava: "Exchange protein directly activated by cAMP 2 is required for corticotropin-releasing hormone-mediated spine loss." in: The European journal of neuroscience, Vol. 50, Issue 7, pp. 3108-3114, (2020) (PubMed).

    Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." in: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).

    Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

  • 抗原

    CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

    别名

    CRHR1

    背景

    CRHR1, CRF-R, corticotropin releasing hormone receptor 1, CRF1, CRFR1, CRH-R1h, CRHR, CRHR1f, corticotropin releasing hormone receptor variant 1h, seven transmembrane helix receptor

    基因ID

    1394, 12921, 58959

    NCBI登录号

    NP_004373

    途径

    Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity
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