Cortactin 抗体 (Intracellular)
Quick Overview for Cortactin 抗体 (Intracellular) (ABIN7043085)
抗原
See all Cortactin (CTTN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 346-359, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Cortactin
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特异性
- Intracellular.
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - identical
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产品特性
- Anti-Cortactin Antibody (ABIN7043085, ABIN7045162 and ABIN7045163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin
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亚型
- IgG
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anti-Cortactin (CTTN) (N-Term) antibody
KO Validated CTTN 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 214-513) antibodyCTTN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Cortactin (CTTN) (pTyr466) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 341-450) antibodyCTTN 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-Cortactin (CTTN) antibodyCTTN 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4C6 unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (AA 1-509) antibodyCTTN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (pTyr421) antibodyCTTN 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cortactin (CTTN) (N-Term) antibodyVerified CTTN 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200-1:1000
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说明
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Negative Control: (ABIN7235169)
Blocking Peptide: (ABIN7235169)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Cortactin (CTTN)
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别名
- CTTN
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背景
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Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN,Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.
Alternative names: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN -
基因ID
- 13043
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NCBI登录号
- NM_138565
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UniProt
- Q60598
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途径
- MAPK Pathway
抗原
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