C5AR1 抗体 (Extracellular)
Quick Overview for C5AR1 抗体 (Extracellular) (ABIN7043082)
抗原
See all C5AR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 16-28, Extracellular
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原理
- A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor
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特异性
- Extracellular, N-terminus
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预测反应
- Human only Not recommended for use with rat,mouse samples
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产品特性
- Anti-Human C5aR1 (extracellular) Antibody (ABIN7043082 and ABIN7043927) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize C5aR from human samples only.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5a anaphylatoxin receptor
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亚型
- IgG
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anti-Complement Component 5a Receptor 1 (C5AR1) (C-Term) antibody
Verified C5AR1 适用: 人 WB, FACS, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E2 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibodyC5AR1 适用: 人, Cow, 兔, 雪貂 WB, FACS, IHC (p), IP 宿主: 小鼠 Monoclonal S5-1 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-36) antibodyC5AR1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) antibodyC5AR1 适用: 人, 兔 FACS, IHC, IHC (p), Func, Neut 宿主: 小鼠 Monoclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (FITC)C5AR1 适用: 人, Cow, 兔, 雪貂 FACS 宿主: 小鼠 Monoclonal S5-1 FITC
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (PE)C5AR1 适用: 人, Cow, 兔, 雪貂 FACS 宿主: 小鼠 Monoclonal S5-1 PE
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (APC)C5AR1 适用: 人, Cow, 兔, 雪貂 FACS 宿主: 小鼠 Monoclonal S5-1 APC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
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别名
- C5AR1
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背景
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C5a1 receptor, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88,The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few.C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10.C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14.Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8.
Alternative names: C5aR1, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin receptor, C5R1, Complement component 5a receptor 1, CD88 -
基因ID
- 728
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NCBI登录号
- NM_001736
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UniProt
- P21730
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途径
- Complement System
抗原
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