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CASR 抗体 (Extracellular, N-Term) (FITC)

The FITC-conjugated 兔多克隆 anti-CASR antibody (ABIN7043019) specifically detects CASR in FACS 和 LCI. The antibody is reactive with 人 samples.

FACS

Cell surface detection of CaSR in live intact human THP-1 monocytic leukemia cells: (black line) Cells. (CASR 抗体 (Extracellular, N-Term) (FITC))

FACS

2 images

产品编号 ABIN7043019

发货至: 中国

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Quick Overview for CASR 抗体 (Extracellular, N-Term) (FITC) (ABIN7043019)

抗原

See all CASR 抗体

CASR (Calcium-Sensing Receptor (CASR))

适用

人

宿主

兔

克隆类型

多克隆

标记

This CASR antibody is conjugated to FITC

应用范围

Flow Cytometry (FACS), Live Cell Imaging (LCI)

Discover our top product CASR Primary Antibody

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AA 216-229, Extracellular, N-Term

原理

A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC

交叉反应

人, 小鼠, 大鼠

预测反应

Rat,mouse,bovine,dog - identical

产品特性

Anti-Calcium Sensing Receptor (extracellular) Antibody (ABIN7043018, ABIN7044161 and ABIN7044162) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize CaSR from human, rat, and mouse samples. \nAnti-Calcium Sensing Receptor (extracellular)-FITC Antibody (ABIN7043018, ABIN7044161 and ABIN7044162)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

纯化方法

Affinity purified on immobilized antigen.

免疫原

(C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

亚型

IgG
anti-Calcium-Sensing Receptor (CASR) (AA 874-1088) antibody
CASR 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (pThr888) antibody
CASR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (AA 121-220) antibody
CASR 适用: Pig WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (C-Term) antibody
CASR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) antibody
CASR 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (AA 926-1078) antibody
CASR 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (AA 216-229), (Extracellular), (N-Term) antibody
KO Validated CASR 适用: 人 WB, IHC, IF, FACS, LCI, IC 宿主: 兔 Polyclonal unconjugated

anti-Calcium-Sensing Receptor (CASR) (AA 260-287) antibody
CASR 适用: 小鼠 WB 宿主: 兔 Polyclonal RB41198 unconjugated

anti-Calcium-Sensing Receptor (CASR) (AA 1-100) antibody (Atto 390)
CASR 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Atto 390

anti-Calcium-Sensing Receptor (CASR) (AA 1-100) antibody
CASR 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: The optimal concentration should be determined by the user
FC: 2.5-5 μg per reaction
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user

说明

Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7235016)

限制

仅限研究用
状态

Lyophilized

溶解方式

50 μL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
抗原

CASR (Calcium-Sensing Receptor (CASR))

别名

CASR

背景

Synonyms: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A
Description: The calcium-sensing receptor (CaSR) is a class C G-protein-coupled receptor encoded by a single copy gene. CaSR plays a major role in the maintenance of a physiological serum ionized Ca2+ concentration by regulating the circulating levels of parathyroid hormone1.The N-terminus of the receptor is comprised of a signal peptide of 19 amino acids. This terminus directs the nascent CaSR polypeptide into the lumen of the endoplasmic reticulum (ER) and is then cleaved. The extracellular domain (ECD) of the mature protein has approximately 600 amino acids, comprised of a bi-lobed Venus-flytrap-like domain (VFT) connected by a cysteine-rich region and a short peptide linker to the seven transmembrane domain (TMD) with an intracellular C-terminal tail of 216 amino acids. CaSR functions as a homodimer with both covalent and noncovalent interactions between the VFT domains of each monomer. Ca2+ binds in the cleft between the two lobes of each VFT causing the lobes to close on one another and the VFT to rotate with transfer of a conformational change to the cysteine-rich region.In the parathyroid gland, up-regulation of CaSR by 1,25-dihydroxyvitamin D [1,25(OH)2D] makes the gland more responsive to extracellular Ca2+ and parathyroid hormone (PTH) secretion is reduced. While somatic mutations in CASR are rare, parathyroid glands from patients with primary or severe uremic secondary hyperparathyroidism have reduced CaSR expression2.

基因ID

846

NCBI登录号

NM_000388

UniProt

P41180

途径

Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis