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- 抗原 See all ADGRB1 products
- ADGRB1 (Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1))
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抗原表位
- AA 97-112, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ADGRB1 antibody is conjugated to FITC
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应用范围
- Live Cell Imaging (LCI), Flow Cytometry (FACS)
- 原理
- A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
- 特异性
- Extracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 交叉反应 （详细）
- The antibody will recognize the intact BAI1 receptor as well as the proteolytically processed N-terminal fragment. This fragment is also known as Vasculostatin (Vstat120).
- 预测反应
- Rat,human - identical
- 产品特性
- Anti-BAI1 (extracellular) Antibody (ABIN7042971 and ABIN7043944) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize BAI1 from rat, mouse, and human samples. \nAnti-BAI1 (extracellular)-FITC Antibody (ABIN7042971 and ABIN7043944)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)GRVRTYQFDSFLESTR, corresponding to amino acid residues 97-112 of mouse BAI1
- 亚型
- IgG
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anti-Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1) (AA 97-112), (Extracellular) antibody
ADGRB1 适用: 小鼠 IHC, LCI, WB, IF, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1) (AA 60-350) antibodyADGRB1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1) antibodyADGRB1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
- 说明
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Negative Control: (ABIN7582044)
Blocking Peptide: (ABIN7234880)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- ADGRB1 (Brain-Specific Angiogenesis Inhibitor 1 (ADGRB1))
- 别名
- ADGRB1 (ADGRB1 产品)
- 背景
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Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF,The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus, various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4.Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5. At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2.These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7.
Alternative names: BAI1, Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF - 基因ID
- 107831
- NCBI登录号
- NM_001702
- UniProt
- Q3UHD1
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