ATP1A2 抗体 (Intracellular)
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- 抗原 See all ATP1A2 抗体
- ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
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抗原表位
- AA 7-19, Intracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATP1A2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase
- 特异性
- Intracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Mouse,human,dog,pig - identical
- 产品特性
- Anti-Alpha 2 Na+/K+ ATPase Antibody (ABIN7042961, ABIN7044639 and ABIN7044640) is a highly specific antibody directed against an epitope of the rat ATP1A2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ATP1A2 from human, rat, and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)REYSPAATTAENG, corresponding to amino acid residues 7-19 of rat ATP1A2
- 亚型
- IgG
- Top Product
- Discover our top product ATP1A2 Primary Antibody
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anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 451-479) antibody
ATP1A2 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19033 unconjugated
anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (N-Term) antibodyATP1A2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 1-70) antibodyATP1A2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) antibodyATP1A2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: (ABIN7234844)
Blocking Peptide: (ABIN7234844)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
- 别名
- ATP1A2 (ATP1A2 产品)
- 背景
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ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2,The Na+/K+ pumps are members of the P-type family of active cation transport proteins. They consume ATP to actively transport Na+ out of and K+ into the cell. Na+ and K+ gradients are required for maintaining membrane potential, cell volume and secondary transport of other solutes thus making these pumps essential for all mammalian cells. The minimum functional unit of the Na+/K+ pump consists of a heterodimer of α- and β- subunits1. The α subunit (also known as ATP1A) has a molecular mass of about 100 kDa and four distinct isoforms have been identified so far. It has ten transmembrane segments, short extracellular loops and larger cytoplasmic regions. There is a large central loop between M4 and M5 composed of 430 amino acid residues and a long N-terminal tail of about 90 amino acid residues. The nucleotide-binding domain (N domain) and phosphorylation domain (P domain) of the pump are formed by the M4/M5 loop while the actuator domain (A domain) is formed by the amino-terminal tail and the M2/M3 loop. The major isoform, α1, is found in most tissues and is the major form in the kidney and in most epithelia cells. The β-subunit is essential for the delivery and insertion of the α-subunit into the cell's membrane, whilst β-subunit delivery to the membrane is not dependent on α-subunit expression2. ATP1A2 mutations that cause diverse functional abnormalities of the Na+/K+ pump have been implicated in the pathogenesis of familial hemiplegic migraine3.
Alternative names: Alpha 2 Na+/K+ ATPase, ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2 - 基因ID
- 24212
- NCBI登录号
- NM_000702
- UniProt
- P06686
- 途径
- Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process
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