Anoctamin 5 抗体 (AA 532-544)
Quick Overview for Anoctamin 5 抗体 (AA 532-544) (ABIN7042929)
抗原
See all Anoctamin 5 (ANO5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 532-544
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原理
- A Rabbit Polyclonal Antibody to Anoctamin-5 Channel
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特异性
- 2nd intracellular loop
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - identical, human - 12,13 amino acid residues identical
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产品特性
- Anti-Anoctamin-5 Antibody is directed against an epitope of mouse ANO5. Anti-Anoctamin-5 Antibody (ABIN7042929, ABIN7044135 and ABIN7044136) can be used in western blot analysis. It has been designed to recognize ANO5 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5
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亚型
- IgG
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anti-Anoctamin 5 (ANO5) (AA 792-819), (C-Term) antibody
ANO5 适用: 小鼠, 人 WB, IHC (p) 宿主: 兔 Polyclonal RB22028 unconjugated
anti-Anoctamin 5 (ANO5) (AA 1-300) antibodyANO5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7234743)
Blocking Peptide: (ABIN7234743)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Anoctamin 5 (ANO5)
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别名
- ANO5
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背景
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ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L,Anoctamins, also known as TMEM16, is a family of ten proteins involved in a variety of functions including phospholipid scrambling, ion transport and the regulation of other membrane proteins. ANO1 (Anoctamin-1), ANO2 and ANO5 function as Ca2+-activated Cl- channels (CaCCs) and play an important role in calcium dependent chloride secretion. All members of the Anoctamin family present high structural homology and consist of eight transmembrane domains and cytosolic N- and C-termini1.The ANO5 gene is widely distributed and expressed in bone, skeletal muscle, cardiac muscle, brain, lung and kidney and also in the mucosal layer of the GI tract and the skeletal muscle layer of the esophagus of mice2.In phylogenetic analysis ANO5 was found to be a close relative of ANO6. ANO6 has an association with phospholipid scramblase activity. Under both physiological and pathological conditions scramblase dissipates the normal asymmetry of the cell membrane by catalyzing the rapid externalization of phospholipids on the cell membrane and thus promoting apoptosis3.Recessive mutations in the ANO5 gene were found to be linked to limb-girdle muscular dystrophies type 2L (LGMD2L) and non-dysferlin Miyoshi myopathy4. LGMD2L is characterized by proximal weakness, asymmetric quadriceps femoris and atrophy of the biceps brachii muscle5.Mutations in the gene are also linked to the Gnathodiaphyseal dysplasia disease, an unusual generalized autosomal dominant disease. Patients with this syndrome show sclerosis of tubular bones, lesions of jawbones, an increased sensitivity for jaw infection and osteomyeletis and fragile bones6.
Alternative names: Anoctamin-5, ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L -
基因ID
- 233246
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NCBI登录号
- NM_213599
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UniProt
- Q75UR0
抗原
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