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- 抗原 See all ACCN5 抗体
- ACCN5 (Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5))
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抗原表位
- AA 284-297, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ACCN5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to ASIC5 Channel
- 特异性
- Extracellular loop
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- Rat,human - 13,14 amino acid residues identical
- 产品特性
- Anti-ASIC5 (extracellular) Antibody (ABIN7042919, ABIN7045260 and ABIN7045261) is a highly specific antibody directed against an epitope of the mouse protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC5 from human, mouse, and rat samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5
- 亚型
- IgG
- Top Product
- Discover our top product ACCN5 Primary Antibody
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anti-Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5) (AA 140-330) antibody
ACCN5 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5) (AA 201-250) antibodyACCN5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5) (AA 388-414), (C-Term) antibodyACCN5 适用: 人 WB 宿主: 兔 Polyclonal RB38141 unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: (ABIN7234716)
Blocking Peptide: (ABIN7234716)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- ACCN5 (Amiloride-Sensitive Cation Channel 5, Intestinal (ACCN5))
- 别名
- ASIC5 (ACCN5 产品)
- 背景
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Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5,ASIC5 (acid sensing ion channel subunit family member 5) is an amiloride-sensitive ion channel belonging to the Degenerin/Epithelial Na+ channel (Deg/ENaC) family, a group of voltage-insensitive cation channels expressed in the nervous system and in several types of epithelial and immune cells.Structurally, ASICs have two transmembrane domains which line the pore of the channel with a relatively short cytoplasmic section, intracellular N- and C-termini and a large extracellular loop. The proton ligand binds to the extracellular domain, composed of 12 β sheets and 7 α helices2,3.ASICs are permeable to sodium, potassium, and sometimes to calcium and are activated by low pH , both intracellular and extracellular. They are responsible for conducting depolarizing inward Na+ currents. ASICs also contribute to excitotoxic neuronal damage during ischemia and local acidosis due to their sensitivity to acid1-3.ASICs are abundantly expressed in mammalian neurons. ASIC5 is mainly expressed in the brain in the cerebellum, specifically in the ventral uvula and nodulus of the vestibulocerebellum. ASIC5 mRNA is also detected in the liver mainly in the luminal membrane of epithelial cholangiocytes that line the bile duct1.ASICs have been linked to numerous neurological diseases including Parkinson's disease, stroke, traumatic brain injury, multiple sclerosis, and epilepsy3.
Alternative names: ASIC5, Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5 - 基因ID
- 58170
- NCBI登录号
- NM_017419
- UniProt
- Q9R0Y1
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