HLA-F 抗体 (PE)
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Quick Overview for HLA-F 抗体 (PE) (ABIN7013919)
抗原
See all HLA-F (HLAF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-HLA-F PE
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特异性
- The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Inclusion body-derived HLA-F heavy chain
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亚型
- IgG1
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anti-HLA Class I alpha F (HLAF) antibody
HLAF 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-HLA Class I alpha F (HLAF) (AA 194-303) antibodyHLAF 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I alpha F (HLAF) antibodyHLAF 适用: 人 WB 宿主: 小鼠 Monoclonal 25GB7425 unconjugated
anti-HLA Class I alpha F (HLAF) (AA 71-120) antibodyHLAF 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I alpha F (HLAF) (AA 110-159) antibodyHLAF 适用: 人, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I alpha F (HLAF) (N-Term) antibodyHLAF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I alpha F (HLAF) (N-Term) antibodyHLAF 适用: 人, Pig, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I alpha F (HLAF) antibodyHLAF 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 1-5 μg/mL, extracellular and intracellular staining.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- HLA-F (HLAF) (HLA Class I alpha F (HLAF))
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别名
- HLA-F
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背景
- Major histocompatibility complex, class I, F,HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types, surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.,CDA12
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基因ID
- 3134
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UniProt
- P30511
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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