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HLA-F 抗体 (PE)

This anti-HLA-F antibody is a 小鼠单克隆 antibody detecting HLA-F in FACS. Suitable for 人.

Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/mL, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/mL, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) whole blood cells. (HLA-F 抗体 (PE))

Flow cytometry multicolor intracellular staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/mL, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/mL, same as anti-HLA-F PE concentration, black). (HLA-F 抗体 (PE))

Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/mL, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/mL, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) whole blood cells. (HLA-F 抗体 (PE))

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μL reagent / 100 μL of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/mL, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/mL, same as anti-HLA-F PE concentration, black). (HLA-F 抗体 (PE))

4 images

产品编号 ABIN7013919

发货至: 中国

Quick Overview for HLA-F 抗体 (PE) (ABIN7013919)

抗原

See all HLA-F (HLAF) 抗体

HLA-F (HLAF) (HLA Class I alpha F (HLAF))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This HLA-F antibody is conjugated to PE

应用范围

Flow Cytometry (FACS)

克隆位点

3D11

原理

Anti-HLA-F PE

特异性

The mouse monoclonal antibody 3D11 recognizes an extracellular epitope of HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.

纯化方法

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Inclusion body-derived HLA-F heavy chain

亚型

IgG1
anti-HLA Class I alpha F (HLAF) antibody
HLAF 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 3D11 unconjugated

anti-HLA Class I alpha F (HLAF) (AA 194-303) antibody
HLAF 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) (AA 110-159) antibody
HLAF 适用: 人, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) (AA 71-120) antibody
HLAF 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) (N-Term) antibody
HLAF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) (N-Term) antibody
HLAF 适用: 人, Pig, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) antibody
HLAF 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated

anti-HLA Class I alpha F (HLAF) (AA 194-303) antibody (FITC)
HLAF 适用: 人宿主: 兔 Polyclonal FITC

anti-HLA Class I alpha F (HLAF) (AA 194-303) antibody (Biotin)
HLAF 适用: 人 ELISA 宿主: 兔 Polyclonal Biotin

anti-HLA Class I alpha F (HLAF) (AA 194-303) antibody (HRP)
HLAF 适用: 人 ELISA 宿主: 兔 Polyclonal HRP

应用备注

Flow cytometry: Recommended dilution: 1-5 μg/mL, extracellular and intracellular staining.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
抗原

HLA-F (HLAF) (HLA Class I alpha F (HLAF))

别名

HLA-F

背景

Major histocompatibility complex, class I, F,HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types, surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.,CDA12

基因ID

3134

UniProt

P30511

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response

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