IgE 抗体 (Biotin)
Quick Overview for IgE 抗体 (Biotin) (ABIN7013917)
抗原
See all IgE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu IgE Biotin
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特异性
- The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.
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纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
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免疫原
- Purified human IgE.
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亚型
- IgG1
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anti-IgE antibody
适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4G7-325 unconjugated
anti-IgE antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 PE
anti-IgE antibody (APC)适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 APC
anti-IgE antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 FITC
anti-IgE antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 PE
anti-IgE (AA 1-421) antibody适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 1-12 μg/mL
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- IgE
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背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
抗原
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