MICA/B 抗体 (Biotin)
Quick Overview for MICA/B 抗体 (Biotin) (ABIN7013906)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-MICA/MICB Biotin
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特异性
- The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of CD314 (NKG2D), and is able to block CD314-mediated activation of NK cells and cytotoxic T cells.
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纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
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免疫原
- Transfected C1R cells expressing MICA
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亚型
- IgG2a
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anti-MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) antibody
MICA/MICB 适用: 人 FACS, IP, IHC 宿主: 小鼠 Monoclonal 6D4 unconjugated
anti-MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) (AA 81-180) antibodyMICA/MICB 适用: 人 IF (cc), IF (p), IHC (fro), ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) antibody (FITC)MICA/MICB 适用: 人 FACS 宿主: 小鼠 Monoclonal 6D4 FITC
anti-MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) antibody (APC)MICA/MICB 适用: 人 FACS 宿主: 小鼠 Monoclonal 6D4 APC
anti-MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB) antibody (PE)MICA/MICB 适用: 人 FACS 宿主: 小鼠 Monoclonal 6D4 PE
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应用备注
- Flow cytometry: Recommended dilution: 1-12 μg/mL.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- MICA/B (MICA/MICB) (MHC Class I Polypeptide-Related Sequence A/B (MICA/MICB))
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别名
- MICA/MICB
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背景
- MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
抗原
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