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Cathepsin B 抗体 (Internal Region)

This anti-Cathepsin B antibody is a 山羊 多克隆 antibody detecting Cathepsin B in WB, IHC, ELISA, FACS 和 IF. Suitable for 人.
产品编号 ABIN7013865
发货至: 中国

Quick Overview for Cathepsin B 抗体 (Internal Region) (ABIN7013865)

抗原

See all Cathepsin B (CTSB) 抗体
Cathepsin B (CTSB)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Cathepsin B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Cathepsin B

    序列

    SDRICIHTNA HVSVE

    特异性

    Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8x kDa according to NP_001899.1). Recommended concentration: 0.5-2 μg/m

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

    限制

    仅限研究用
  • 缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Cathepsin B (CTSB)

    别名

    CTSB

    背景

    CTSB, cathepsin B, APPS, CPSB, APP secretase, amyloid precursor protein secretase, cathepsin B1, cysteine protease

    基因ID

    1508

    途径

    Activation of Innate immune Response, Toll-Like Receptors Cascades
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