Caspase 3 p12 (Cleaved-Asp175) 抗体
(1 validation)Quick Overview for Caspase 3 p12 (Cleaved-Asp175) 抗体 (ABIN7251292)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cleaved-Asp175
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产品特性
- Polyclonal Antibody
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纯化方法
- Affinity purification
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免疫原
- Synthesized peptide derived from the Internal region of human Caspase-3 p12
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亚型
- IgG
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anti-Caspase 3 p12 (Asp175), (cleaved) antibody
适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Caspase 3 p12 antibodyKD Validated 适用: 人, 小鼠, 大鼠 WB, IF, IHC, ICC, IP 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
- WB 1:500-2000, IHC 1:50-300, IF 1:50-300
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限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104628
- 日期
- 2025.03.15
- 抗原
- Caspase 3 p12
- Lot Number
- DX05PXZ02179
- Method validated
- Immunohistochemistry
- Positive Control
The expression of caspase 3 at P5 was already attested in our laboratory (Lossi et al., 2004).
- Negative Control
A one control slice was processed for each experimental procedure, omitting the primary antibody.
- Notes
Reference:
- Lossi L., Tamagno I. and Merighi A. (2004) “Molecular morphology of neuronal apoptosis: analysis of Caspase 3 activation during postnatal development of mouse cerebellar cortex.” J Mol Histol, 35(6):621-9.
- Primary Antibody
- ABIN7251291
- Secondary Antibody
- anti-rabbit Alexa Fluor 488 (Invitrogen. Lot 2541675)
- Full Protocol
- Sample analyzed: mouse brain at post-natal day (P) 5-6
- Paraffin-embedded slices (7 µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
- Sections were blocked in ovalbumin 1% 1 hour at room temperature
- Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6)
- 3x5 minutes washing in 0.01M PBS
- Sections were incubated with the primary antibody at the following dilutions of 1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent)
- 3x5 minutes washing in 0.01M PBS
- Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
- 3x5 minutes washing in 0.01M PBS
- Nuclear counterstaining was performed with DAPI 1µg/mL, 2 minutes
- 2x5 minutes washing in 0.01M PBS
- Specimens were then mounted in Fluoroshield mounting medium (Sigma)
- Experimental Notes
The antibody works in IHC-P at concentrations from 1:50-1:200.
生效 #104628 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Expression of cleaved caspase 3 (cCASP3) in P6 mouse cerebellum. Immunoreactive granule cells after immunostaining with ABIN7251291 and nuclear counterstaining with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI). EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex.]()
Expression of cleaved caspase 3 (cCASP3) in P6 mouse cerebellum. Immunoreactive granule cells after immunostaining with ABIN7251291 and nuclear counterstaining with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI). EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex.
Full Methods -
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- Caspase 3 p12
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别名
- CASP3 p12
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背景
- Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
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分子量
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Observed_MW: 20+35 kDa
Calculated_MW: 32 kDa
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UniProt
- P42574
抗原
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