FLIP 抗体 (AA 210-460)
Quick Overview for FLIP 抗体 (AA 210-460) (ABIN7439762)
抗原
See all FLIP (CFLAR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
 - AA 210-460
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原理
 - Polyclonal Antibody to CASP8 And FADD Like Apoptosis Regulator (CFLAR)
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特异性
 - The antibody is a rabbit polyclonal antibody raised against CFLAR. It has been selected for its ability to recognize CFLAR in immunohistochemical staining and western blotting.
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纯化方法
 - Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
 - Recombinant CASP8 And FADD Like Apoptosis Regulator (CFLAR) corresdonding to Asn210~Gly460 with N-terminal His Tag
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亚型
 - IgG
 
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应用备注
 - Western blotting: 0.5-2 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 500 μg/mL
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缓冲液
 - 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
 - ProClin
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注意事项
 - This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
 - 24 months
 
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- FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))
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别名
 - CFLAR
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背景
 - CASH, CASP8AP1, CLARP, Casper, FLAME1, FLIP, I-FLICE, MRIT, USURPIN, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, Caspase-eight-related protein, FADD-like antiapoptotic molecule 1
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UniProt
 - O35732
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途径
 - Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
 
抗原
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