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GCLC 抗体 (AA 435-636)

This 兔 多克隆 anti-GCLC antibody specifically detects GCLC in WB, IHC, IP 和 ICC. The antibody is reactive with 小鼠 samples.
产品编号 ABIN7435583
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GCLC 抗体 (AA 435-636) (ABIN7435583)

抗原

See all GCLC 抗体
GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

适用

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小鼠

宿主

  • 65
  • 4

克隆类型

  • 53
  • 16
多克隆

标记

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This GCLC antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 435-636

    原理

    Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)

    特异性

    The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining and western blotting.

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    Recombinant Glutamate Cysteine Ligase, Catalytic (GCLC) corresdonding to Asp435~Ser636 with N-terminal His Tag

    亚型

    IgG
  • 应用备注

    Western blotting: 0.5-2 μg/mL,1:450-1800Immunohistochemistry: 5-20 μg/mL,1:45-180Immunocytochemistry: 5-20 μg/mL,1:45-180Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.9 mg/mL

    缓冲液

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    24 months
  • 抗原

    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

    别名

    GCLC

    背景

    GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

    UniProt

    P97494

    途径

    Cell RedoxHomeostasis
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