Reelin 抗体 (AA 3143-3461)
(1 validation)Quick Overview for Reelin 抗体 (AA 3143-3461) (ABIN7435136)
抗原
See all Reelin (RELN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 3143-3461
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原理
- Polyclonal Antibody to Reelin (RELN)
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特异性
- The antibody is a rabbit polyclonal antibody raised against RELN. It has been selected for its ability to recognize RELN in immunohistochemical staining and western blotting.
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交叉反应
- 人, 大鼠
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Reelin (RELN) corresdonding to Glu3143~Pro3461 (Accession # Q60841)
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亚型
- IgG
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anti-Reelin (RELN) (N-Term) antibody
RELN 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 26-190) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Reelin (RELN) (AA 2978-3373) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Reelin (RELN) (AA 26-254) antibodyRELN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) antibodyMicroarray verified RELN 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB299520 unconjugated
anti-Reelin (RELN) (AA 3142-3460) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 2086-2296) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 26-190) antibodyRELN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) (AA 2400-2610) antibodyRELN 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Reelin (RELN) antibodyRELN 适用: 人, 大鼠, 小鼠 IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104517
- 日期
- 2024.01.24
- 抗原
- Reelin
- Lot Number
- A20230619938
- Method validated
- Immunohistochemistry
- Positive Control
Postnatal day 5 and adult (> 2 months) CD1 mouse brain fixed in 4% paraformaldehyde.
- Negative Control
One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.
- Notes
Passed. The Reelin antibody antibody (AA 3143-3461) ABIN7435136 works in for IHC-P at 1:100 concentration with microwave antigen retrieval.
- Primary Antibody
- ABIN7435136
- Secondary Antibody
- anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific A11037, lot # 2439060)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix of samples in the same fixative for an additional 2 h at RT
- Wash, dehydrate, and embed samples in paraffin wax
- Wash several times with 0.01 M PBS
- Cut brain with a microtome into 6 µm sections and mount on glass slides
- After paraffin removal sections were processed for microwave antigen retrieval for 10 minutes (95-100° C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 minutes each; followed by PBS for 5 minutes)
- Incubate sections for 1 h at RT in a humid chamber in PBS containing 1% ovalbumin (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites
- Incubate sections with primary rabbit anti-Reelin (antibodies-online, ABIN7435136, lot A20230619938) diluted 1:50 and 1:100 in PBS-BSA-PLL (Sigma, P1524) ON at RT in a humid chamber
- Wash sections 3x 5 min with 0.01 M PBS
- Incubate sections with anti-rabbit secondary antibody Alexa Fluor 594, 1:500, in 0.1M PBS (Thermo Fisher A11037, lot # 2439060) for 1 h at RT
- Wash sections 3x 5 min with 0.01 M PBS
- Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V)
- Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 40x magnification
- Experimental Notes
生效 #104517 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x]()
Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x
Full Methods -
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- Reelin (RELN)
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别名
- RELN
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背景
- RL
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UniProt
- Q60841
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途径
- Synaptic Membrane
抗原
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