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GAPDH 抗体 (AA 154-324)

This anti-GAPDH antibody is a 兔 多克隆 antibody detecting GAPDH in WB, IHC, IP, ICC 和 FACS. Suitable for 人.
产品编号 ABIN7438157
发货至: 中国

Quick Overview for GAPDH 抗体 (AA 154-324) (ABIN7438157)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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宿主

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克隆类型

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多克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

    • 35
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    AA 154-324

    原理

    Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

    特异性

    The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

    交叉反应

    小鼠, 大鼠

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    Recombinant Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) corresdonding to Thr154~Val324 with N-terminal His Tag

    亚型

    IgG
  • 应用备注

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    24 months
  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

    UniProt

    P04406
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