Estrogen Receptor alpha 抗体 (AA 233-483)
Quick Overview for Estrogen Receptor alpha 抗体 (AA 233-483) (ABIN7433072)
抗原
See all Estrogen Receptor alpha (ESR1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 233-483
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原理
- Polyclonal Antibody to Estrogen Receptor Alpha (ERa)
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特异性
- The antibody is a rabbit polyclonal antibody raised against ERa. It has been selected for its ability to recognize ERa in immunohistochemical staining and western blotting.
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交叉反应
- 人, 大鼠
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Estrogen Receptor Alpha (ERa) corresdonding to Ile233~Leu483 with N-terminal His and GST Tag
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亚型
- IgG
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应用备注
- Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin, Sodium azide
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注意事项
- This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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别名
- Estrogen Receptor Alpha
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背景
- ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1
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UniProt
- P19785
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途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
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