PKM2 抗体 (AA 141-248)
Quick Overview for PKM2 抗体 (AA 141-248) (ABIN7432133)
抗原
See all PKM2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 141-248
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原理
- Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
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特异性
- The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- Recombinant Pyruvate kinase isozymes M2 (PKM2) corresdonding to Lys141~Ala248 with N-terminal His Tag
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亚型
- IgG
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应用备注
- Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunofluorescence:5-20 μg/mL,Flow cytometry:20 μg/mL,Optimal working dilutions must be determined by end user.,
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 24 months
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- PKM2 (Pyruvate Kinase M2 (PKM2))
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别名
- PKM2
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背景
- M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein, Pyruvate Kinase, Muscle
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UniProt
- P14618
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途径
- Warburg Effect
抗原
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