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HMGB1 抗体 (AA 1-215)

This anti-HMGB1 antibody is a 小鼠 单克隆 antibody detecting HMGB1 in WB, IHC, ICC 和 IP. Suitable for 人.
产品编号 ABIN7428749
发货至: 中国

Quick Overview for HMGB1 抗体 (AA 1-215) (ABIN7428749)

抗原

See all HMGB1 抗体
HMGB1 (High Mobility Group Box 1 (HMGB1))

适用

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宿主

  • 156
  • 44
  • 2
  • 2
  • 2
  • 1
  • 1
小鼠

克隆类型

  • 144
  • 64
单克隆

标记

  • 118
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  • 6
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  • 2
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This HMGB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

C1
  • 抗原表位

    • 32
    • 16
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    • 11
    • 9
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    • 2
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    • 1
    • 1
    • 1
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    • 1
    • 1
    AA 1-215

    原理

    Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1)

    特异性

    The antibody is a mouse monoclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.

    纯化方法

    Protein A + Protein G affinity chromatography

    免疫原

    Recombinant High Mobility Group Protein 1 (HMGB1) corresdonding to Met1~Glu215 with N-terminal His Tag

    亚型

    IgG1 kappa
  • 应用备注

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    24 months
  • 抗原

    HMGB1 (High Mobility Group Box 1 (HMGB1))

    别名

    HMGB1

    背景

    HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein

    UniProt

    P09429

    途径

    p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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