Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
RHOG antibody is human, mouse and rat reactive.
纯化方法
RHOG antibody is affinity chromatography purified via peptide column.
免疫原
RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG. The immunogen is located within amino acids 40 - 90 of RHOG.
RHOG
适用: 人
FACS, IF (cc), IF (p)
宿主: 兔
Polyclonal
Cy5.5
应用备注
RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
RHOG antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
RHOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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背景
The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).