MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C
纯化方法
MAP1LC3B antibody is affinity chromatography purified via peptide column.
免疫原
MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B.
MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
MAP1LC3B antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
MAP1LC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
LC3B (MAP1LC3B)
(Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).