NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist, this antibody will detect both isoforms of NCBP1.
纯化方法
NCBP1 Antibody is affinity chromatography purified via peptide column.
免疫原
NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1. The immunogen is located within amino acids 420 - 470 of NCBP1.
NCBP1
适用: 小鼠
ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
NCBP1 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
NCBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
NCBP1
(Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))
别名
NCBP1
背景
NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).