Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
This antibody is predicted to not cross-react with other Ras-related proteins
纯化方法
RAB21 Antibody is affinity chromatography purified via peptide column.
免疫原
RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21. The immunogen is located within the last 50 amino acids of RAB21.
RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
RAB21 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
RAB21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MGC68816 antibody, RAB21 antibody, 9630024B22 antibody, RAB21, member RAS oncogene family antibody, RAB21, member RAS oncogene family L homeolog antibody, RAB21 antibody, rab21.L antibody, rab21 antibody, Rab21 antibody
背景
RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α,-tail of endocytosed integrin.