Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
特异性
ECRG4 antibody is predicted to not cross-react with other ECRG family members.
纯化方法
ECRG4 Antibody is affinity chromatography purified via peptide column.
免疫原
ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4. The immunogen is located within the last 50 amino acids of ECRG4.
ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ECRG4 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ECRG4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
C2orf40
(Chromosome 2 Open Reading Frame 40 (C2orf40))
ECRG4 antibody, ecrg4 antibody, ecrg4-A antibody, Ecrg4 antibody, chromosome 2 open reading frame, human C2orf40 antibody, chromosome 13 open reading frame, human C2orf40 antibody, chromosome 11 open reading frame, human C2orf40 antibody, chromosome 2 open reading frame 40 antibody, chromosome 2 open reading frame 40 L homeolog antibody, RIKEN cDNA 1500015O10 gene antibody, similar to RIKEN cDNA 1500015O10 antibody, C2H2orf40 antibody, C13H2orf40 antibody, C11H2orf40 antibody, C2orf40 antibody, c2orf40.L antibody, 1500015O10Rik antibody, RGD1305645 antibody
背景
ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.