ROCK2 抗体 (AA 920-970)
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Quick Overview for ROCK2 抗体 (AA 920-970) (ABIN6991526)
抗原
See all ROCK2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 920-970
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特异性
- Three isoforms of ROCK2 are known to exist, this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
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纯化方法
- ROCK2 Antibody is affinity chromatography purified via peptide column.
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免疫原
- ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2. The immunogen is located within amino acids 920 - 970 of ROCK2.
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亚型
- IgG
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应用备注
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ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- ROCK2 Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- ROCK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
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别名
- ROCK2
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背景
- ROCK2 Antibody: ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
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分子量
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Predicted: 153 kDa
Observed: 160 kDa -
基因ID
- 9475
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NCBI登录号
- NP_004841
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UniProt
- O75116
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途径
- Microtubule Dynamics, WNT signaling, Tube Formation
抗原
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