RNF8 抗体 (C-Term)
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- 抗原 See all RNF8 抗体
- RNF8 (Ring Finger Protein 8 (RNF8))
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抗原表位
- AA 330-380, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RNF8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 特异性
- At least three isoforms of RNF8 are known to exist, this antibody will detect all three isoforms.
- 纯化方法
- RNF8 Antibody is affinity chromatography purified via peptide column.
- 免疫原
- RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. The immunogen is located within amino acids 330 - 380 of RNF8.
- 亚型
- IgG
- Top Product
- Discover our top product RNF8 Primary Antibody
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anti-Ring Finger Protein 8 (RNF8) (AA 1-485) antibody
RNF8 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (N-Term) antibodyVerified RNF8 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (C-Term) antibodyRNF8 适用: 人, 小鼠, 大鼠, Cow, Pig, 马, 兔, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 1-260) antibodyRNF8 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 1-485) antibodyRNF8 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 351-400) antibodyRNF8 适用: 人, 小鼠, 大鼠, Cow, Pig, 马, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) antibodyRNF8 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 335-364) antibodyRNF8 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 1-485) antibodyRNF8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 8 (RNF8) (AA 1-260) antibodyRNF8 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunoflourescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- RNF8 Antibody is supplied in PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C,4 °C
- 储存方法
- RNF8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- 抗原
- RNF8 (Ring Finger Protein 8 (RNF8))
- 别名
- RNF8 (RNF8 产品)
- 别名
- hRNF8 antibody, 3830404E21Rik antibody, AIP37 antibody, RNF8 antibody, rnf8 antibody, fc66f09 antibody, wu:fc66f09 antibody, zgc:55936 antibody, zgc:77313 antibody, ring finger protein 8 antibody, ring finger protein 8, E3 ubiquitin protein ligase S homeolog antibody, ring finger protein 8, E3 ubiquitin protein ligase L homeolog antibody, ring finger protein 8, E3 ubiquitin protein ligase antibody, RNF8 antibody, Rnf8 antibody, rnf8.S antibody, rnf8.L antibody, rnf8 antibody
- 背景
- RNF8 Antibody: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.
- 分子量
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Predicted: 46, 53 kDa
Observed: 50 kDa - 基因ID
- 9025
- NCBI登录号
- NP_003949
- UniProt
- O76064
- 途径
- Production of Molecular Mediator of Immune Response
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