Hrk Antibody is affinity chromatography purified via peptide column.
免疫原
HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk. The immunogen is located within the last 50 amino acids of Hrk.
HRK
适用: 小鼠, 大鼠
IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 488
应用备注
Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Hrk Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
Hrk antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
HRK
(Harakiri, BCL2 Interacting Protein (Contains Only BH3 Domain) (HRK))
Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.