ATG7 抗体 (N-Term)
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北京 101111
Quick Overview for ATG7 抗体 (N-Term) (ABIN6990576)
抗原
See all ATG7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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纯化方法
- APG7 Antibody is affinity chromatography purified via peptide column.
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免疫原
- APG7 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human APG7. The immunogen is located within the first 50 amino acids of APG7.
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亚型
- IgG
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应用备注
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APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 4 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- APG7 Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- APG7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- ATG7 (ATG7 Autophagy Related 7 (ATG7))
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别名
- APG7
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背景
- APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme, this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
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分子量
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Predicted: 77 kDa
Observed: 77 kDa -
基因ID
- 10533
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NCBI登录号
- NP_006386
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UniProt
- O95352
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途径
- Response to Water Deprivation, Autophagy
抗原
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