Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
特异性
Depending on cell lines or tissues used, other cleavage products may be observed.
纯化方法
Caspase-5 Antibody is affinity chromatography purified via peptide column.
免疫原
Caspase-5 antibody was raised against a 14 amino acid synthetic peptide from the center of human Caspase-5. The immunogen is located within amino acids 170 - 220 of Caspase-5.
Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Caspase-5 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
Caspase-5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Caspase-5 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1β, and IL-18. Caspase-5 can interact with caspase-1, both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1β,. Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma.