PUMA 抗体 (C-Term, Isoform 1)
Quick Overview for PUMA 抗体 (C-Term, Isoform 1) (ABIN6990494)
抗原
See all PUMA (BBC3) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term, Isoform 1
-
特异性
- A lower band at approximately 16 kDa was detected in Daudi and K562 cells, which may represent the PUMA isoform 2.
-
纯化方法
- PUMA Antibody is affinity chromatography purified via peptide column.
-
免疫原
- Anti-PUMA antibody was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus human PUMA isoform 1. The immunogen is located within the last 50 amino acids of PUMA.
-
亚型
- IgG
-
-
anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibody
BBC3 适用: 人, 大鼠, Pig WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB01353 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibodyBBC3 适用: 人, 小鼠 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 186-199) antibodyBBC3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibodyBBC3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D10 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibodyBBC3 适用: 人, 小鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 2-16) antibodyBBC3 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibodyBBC3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
WB: 1-4 μ,g/mL, IF: 2 μ,g/mL, ICC: 1 μ,g/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry and Immunofluorescence in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PUMA Antibody is supplied in PBS containing 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C,4 °C
-
储存方法
- PUMA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
-
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
-
别名
- PUMA
-
背景
- PUMA Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53 (1). The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (2). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
-
分子量
- 23 kDa
-
基因ID
- 27113
-
NCBI登录号
- NP_055232
-
UniProt
- Q96PG8
-
途径
- p53 Pathway, Positive Regulation of Endopeptidase Activity
抗原
-
