DFFA 抗体 (C-Term)
Quick Overview for DFFA 抗体 (C-Term) (ABIN6990267)
抗原
See all DFFA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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纯化方法
- ICAD Antibody is affinity chromatography purified via peptide column.
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免疫原
- ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD. The immunogen is located within the last 50 amino acids of ICAD.
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亚型
- IgG
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anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibody
DFFA 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 51-305) antibodyDFFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 2-21) antibodyDFFA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 231-331) antibodyDFFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 179-331) antibodyDFFA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2B10C8 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 19-262) antibodyDFFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibodyDFFA 适用: 人, 猴 WB, IF 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibodyDFFA 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- ICAD Antibody is supplied in PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- ICAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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别名
- ICAD
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背景
- ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
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分子量
- 45 kDa
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基因ID
- 13347
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UniProt
- O54786
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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