DFFA 抗体 (N-Term)
Quick Overview for DFFA 抗体 (N-Term) (ABIN6990255)
抗原
See all DFFA 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
纯化方法
- DFF45 Antibody is Antibody is DEAE purified.
-
免疫原
- DFF45 antibody was raised against a 20 amino acid peptide near the amino terminus of human DFF45. The immunogen is located within the first 50 amino acids of DFF45.
-
亚型
- IgG
-
-
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibody
DFFA 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 51-305) antibodyDFFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 2-21) antibodyDFFA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 231-331) antibodyDFFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 179-331) antibodyDFFA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2B10C8 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 19-262) antibodyDFFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibodyDFFA 适用: 人, 猴 WB, IF 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibodyDFFA 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
DFF45 antibody can be used for detection of DFF45, DFF35, and one the cleaved fragment by Western blot at 1:1000 to 1:2000 dilution. 45 and 35 kDa bands can be detected in non-apoptotic cells.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- DFF45 Antibody is supplied in PBS containing 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C,4 °C
-
储存方法
- DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
-
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
-
别名
- DFF45
-
背景
- DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
-
分子量
- 45 and 35 kDa
-
基因ID
- 1676
-
NCBI登录号
- NP_004392
-
UniProt
- O00273
-
途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
-
