TET1 抗体 (C-Term)
Quick Overview for TET1 抗体 (C-Term) (ABIN6972860)
抗原
See all TET1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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产品特性
- Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Tet1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples.
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a peptide within the C-terminal region of mouse Tet1.
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亚型
- IgG
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anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 2038-2136) antibody
TET1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1700-1800) antibodyTET1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (AA 1845-1879), (C-Term) antibodyTET1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB52496 unconjugated
anti-Tet Methylcytosine Dioxygenase 1 (TET1) (C-Term) antibodyTET1 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))
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别名
- Tet1
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分子量
- 215 kDa
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NCBI登录号
- NP_081660
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途径
- Stem Cell Maintenance, Warburg Effect
抗原
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